Ungakhetha njani izinto ezityiwayo zePCR/qPCR?

I-PCR yindlela enovakalelo nesebenzayo yokukhulisa ikopi enye yolandelelwano lwe-DNA ekujoliswe kuyo kwizigidi zeekopi ngexesha elifutshane. Ke ngoko, izinto ezityiwayo zeplastiki zokuphendula kwe-PCR kufuneka zingabinazingcolisi kunye nezithinteli, ngelixa zinekhwalithi ephezulu enokuqinisekisa eyona mpembelelo ilungileyo yePCR. Izinto zeplastiki zePCR ezisetyenziswayo zifumaneka ngokobukhulu obahlukeneyo kunye neefomathi, kwaye ukwazi iimpawu ezifanelekileyo zeemveliso kuya kukunceda ekukhetheni izinto ezisetyenziswayo zeplastiki ezifanelekileyo kwi-PCR kunye nedatha ye-qPCR.

Ukuqulunqwa kunye neempawu zePCR ezisetyenziswayo

1.Izinto eziphathekayo
Izinto ezisetyenziswayo ze-PCR ngokuqhelekileyo zenziwe nge-polypropylene, ene-inert eyaneleyo yokumelana nokutshintsha kobushushu obukhawulezayo ngexesha lokuhamba ngebhayisikile eshushu kunye nokunciphisa ukuthathwa kwezinto ezisebenzayo ukuze kuqinisekiswe iziphumo ezilungileyo ze-PCR. I-medical-grade, umgangatho ophezulu we-polypropylene izinto eziluhlaza kufuneka zisetyenziswe ngexesha lokuvelisa kwaye zenziwe kwiKlasi ye-100,000 yokucoceka. Imveliso kufuneka ingabinayo i-nuclease kunye nongcoliseko lwe-DNA ukunqanda ukuphazamisana nesiphumo sovavanyo lokukhulisa i-DNA.

2.Umbala
Iipleyiti zePCRkwayeIityhubhu zePCRzifumaneka ngokubanzi kwi transparent and white.

• Uyilo lobungqimba obufanayo bodonga luya kubonelela ngokutshintshwa kobushushu obuhambelanayo kwiisampuli eziphendulayo.

• Ukugqithiswa okuphezulu kwe-optical ukuqinisekisa ukuhanjiswa kwesignali ye-fluorescence kunye nokuphazamiseka okuncinci.

• Kwimifuniselo ye-qPCR, umngxuma omhlophe uthintele ukuphinda-phinda umqondiso we-fluorescence kunye nokufunxwa kwayo ngemodyuli yokufudumeza.

3.Ukufomatha
I-PCR Plate "isiketi" sijikeleze ibhodi. I-skirt inika uzinzo olungcono kwinkqubo yokubhobhoza xa inkqubo yokusabela yakhiwe, kwaye ibonelele amandla angcono omatshini ngexesha lonyango oluzenzekelayo. Ipleyiti yePCR inokwahlulwa ibekho isiketi, isiketi esisiqingatha kunye nesiketi esigcweleyo.

• Ipleyiti ye-PCR engekho-skirt elahlekileyo ejikeleze ipleyiti, kwaye le fomu yepleyiti yokuphendula inokutshintshwa kwisixhobo esikhulu se-PCR kunye neemodyuli ze-PCR yexesha langempela, kodwa kungekhona kwizicelo ezizenzekelayo.

• I-semi-skirt ye-PCR ipleyiti inomphetho omfutshane ojikeleze umda weplate, inikezela ngenkxaso eyaneleyo ngexesha lokubhobhoza kunye namandla omatshini wokuphatha i-robotic.

• Ipleyiti ye-PCR ene-skirt epheleleyo inomphetho ogubungela ubude beplate. Le fom yepleyiti ifanelekile kwimisebenzi ezenzekelayo, enokuthi ikhuseleke kwaye izinzile. Isiketi esigcweleyo sikwanyusa amandla oomatshini, isenza ukuba silungele ukusetyenziswa kunye neerobhothi ekuhambeni komsebenzi okuzenzekelayo.

I-tube ye-PCR ifumaneka kwi-tube enye kunye ne-8-strips, efanelekileyo kakhulu kwi-lowput throughput ye-PCR/qPCR. Isigqubuthelo esisicaba siyilelwe ukuququzelela ukubhala, kwaye ukuhanjiswa kokuthembeka okuphezulu kophawu lwe-fluorescence kunokuqondwa ngcono yi-qPCR.

• Ityhubhu enye inika ukuguquguquka ukuseta inani elichanekileyo lokuphendula. Kwimithamo emikhulu yokusabela, ityhubhu enye kwi-0.5 mL ubukhulu iyafumaneka.
• I-tube ye-8-strips ene-caps ivula kwaye ivale iibhubhu zesampuli ngokuzimeleyo ukukhusela isampuli.

4.ukutywinwa
Isigqubuthelo sombhobho kunye nefilimu yokutywina kufuneka itywine ngokupheleleyo ityhubhu kunye nepleyiti ukuthintela ukukhuphuka kwesampulu ngexesha lokujikeleza kwe-thermal. Isitywina esiqinileyo sinokuqondwa ngokusebenzisa i-movie scraper kunye nesixhobo sokushicilela.

• Imithombo yeplate ye-PCR inomphetho ophakamileyo ojikelezayo. Olu yilo lunceda ukutywina ipleyiti ngefilimu yokutywina ukuthintela ukuvela komphunga.
• Ukumakishwa kwe-alphanumeric kwipleyiti ye-PCR kuya kunceda ukuchonga amaqula ngamnye kunye nezikhundla zeesampuli ezihambelanayo. Iileta ezinamaqabunga zivame ukuprintwa kumhlophe okanye kumnyama, kwaye kwizicelo ezizenzekelayo, ukubhalwa kweleta kunenzuzo ngakumbi ukutywina imiphetho yangaphandle yepleyiti.

5.Flux isicelo

Uvavanyo lovavanyo lwe-PCR / qPCR lunokumisela ukuba loluphi uhlobo lwezinto ezityiwayo zeplastiki ekufuneka zisetyenziselwe esona siphumo sonyango. Ukusetyenziswa kwe-throughput ephantsi ukuya kwimodareyitha, iibhubhu zihlala zifanelekile ngakumbi, ngelixa iiplate zinqweneleka ngakumbi kwi-medium-to-high throughput experimental. Iipleyiti nazo zenzelwe ukuqwalasela ukuguquguquka kwe-flux, enokwahlulwa ibe ngumcu omnye.

Ukuqukumbela, njengenxalenye ebalulekileyo yolwakhiwo lwenkqubo yePCR, izinto zeplastiki ezisetyenziswayo zibalulekile kwimpumelelo yovavanyo kunye nokuqokelelwa kwedatha, ngakumbi kusetyenziso lokuhamba komsebenzi oluphakathi ukuya phezulu.

Njengomthengisi waseTshayina wezinto ezisetyenziswayo zeplastiki ezizenzekelayo, iCotaus ibonelela ngeengcebiso zepipette, i-nucleic acid, uhlalutyo lweprotheyini, inkcubeko yeeseli, ukugcinwa kwesampulu, ukutywinwa, ichromatography, njl.

Cofa kwisihloko semveliso ukujonga iinkcukacha zemveliso yePCR.

Umbhobho wePCR ;Ipleyiti yePCR