Indlela yokukhetha incam yepipette jikelele?

IiPipettes zizixhobo zaselabhoratri ezisetyenziselwa ukuphatha iisampuli zolwelo. Phantse zonke iipayipi zifuna iingcebiso ze-pipette zokwenza umsebenzi wabo ojoliswe kuwo. Ngokwendalo, ukukhetha uhlobo olululo lwe-universal pipette tip kuyimfuneko.

Incam yePipette eyenziwe ngepolypropylene enyulu yeyona ixhaphakileyo kwaye ihambelana nokusingqongileyo. Iingcebiso ze-pipette zihlulwe zibe ziintlobo ezininzi ngokusekelwe kwimisebenzi yazo kunye neempawu.

1.Uhlobo lokukhetha incam yepipette:
● Iingcebiso zesihluzi
Imibhobho idala ii-aerosols ezithwala umngcipheko wongcoliseko olunqamlezayo. Iingcebiso zokucoca zifakwe isihluzi ukuphepha ukubunjwa kwe-aerosols. Olu hlobo lwe-pipette luye lwaba luncedo kwi-PCR (i-polymerase chain reaction), ukuphatha i-RNA / DNA, i-radio-labeled, i-infectious, kunye neesampuli eziguquguqukayo.
●Iingcebiso zokugcinwa okuphantsi
Ezi ngcebiso zibamba ulwelo oluncinci kunezo zisetyenziswa ngokubanzi, ezinceda ukugcina iisampulu/izixhobo zokusebenza. Ezi ngcebiso zilungile kwiisampuli ze-viscous kunye nezigxininiswe kakhulu. Nangona kunjalo, ezi ngcebiso ze-pipette zibiza kakhulu. Ezi zilungele i-electrophoresis, uhlalutyo lweprotheyini, ukulandelelana, okanye naluphi na uvavanyo olusebenzisa i-viscous kunye ne-concentrated liquids.
●Iingcebiso ezinde
Ngamanye amaxesha ii-reagents okanye iisampulu zinomthamo omncinci kwaye zisezantsi kwesikhongozeli. Kuthetha ukubeka kuphela i-pipette tip kodwa kunye ne-shaft ye-pipette ngaphakathi kwesitya. Oku kwandisa umngcipheko wongcoliseko, ngoko ke ukusebenzisa incam yepipette emide kunesiqhelo yeyona nto ilungileyo endaweni.
●Iingcebiso ezimfutshane
Iingcebiso ezandisiweyo ziba nzima xa iisampulu zitsalwa okanye zibekwe kumaqula amancinane. Ngoko ke, ukusebenzisa iingcebiso ezimfutshane kunye ne-pipette ye-multichannel iyona nto ifanelekileyo. Ngokunjalo, ukubhobhoza ngeengcebiso ezide kunokunciphisa izandla kwaye kufuna indawo ebanzi yebhentshi. Ke, ukutshintshela kwiingcebiso ezimfutshane ukuze ugweme le miqathango yeyona ndlela ilungileyo.
●Wide Bore Tips
Ngamanye amaxesha iisampulu eziphathwa yilabhoratri zinokuba buthathaka kwaye ziwohloke ngelixa zigqithiselwa ukusuka kwindawo emxinwa yeengcebiso eziqhelekileyo. Ke, ukusebenzisa iingcebiso nge-orifice ebanzi yeyona ndlela ilungileyo yokuphatha iisampuli ezibandakanya iiseli okanye ezixinene kakhulu.

Iingcebiso zeCotaus pipette

2.Iinqobo zokugweba zokukhetha iTip yePipette eLungileyo jikelele:
Iikhrayitheriya eziphambili zokukhetha incam yepipette efanelekileyo luvavanyo oluqhubayo kwibhubhoratri yakho. Iingcebiso zokucoca iintsholongwane ziyimfuneko ukuba uzama iimvavanyo zemolekyuli elabhoratri. Kunye novavanyo, kukho iindlela ezininzi ekufuneka uziqwalasele ngaphambi kokuthenga iingcebiso. Zimi ngolu hlobo lulandelayo:
●Imiqulu yolwelo oluphathwayo
Umthamo weisampulu zolwelo okanye ii-reagents zihluka ngokubanzi kwiilebhu. Kwibhubhoratri, kungcono ukuba neengcebiso zobukhulu obuhlukeneyo kunye neenjongo.
●I-Pipette esetyenzisiweyo
Ukuba uphethe iisampuli ezininzi ngexesha elincinci, unokusebenzisa i-pipette ye-multichannel. Sekunjalo, iilabhoratri eziqhelekileyo zisebenzisa i-micropipettes, ngoko ukuthenga iingcebiso ezifanelekileyo kuzo zombini iindidi ngobuninzi kunoqoqosho ngakumbi.
●Inkampani ekhethwayo
Ukuba ukhetha ingcebiso yokuvala inzala, kufuneka ukhangele inkampani ebonelela ngesatifikethi sokuvala inzala. Ngokufanayo, iingcebiso ezingezizo iintsholongwane zinokusetyenziswa kwakhona ukuba inkampani ithi iyakwazi ukwenzeka.
●Uhlahlo lwabiwo-mali
Uhlahlo lwabiwo-mali lwakho luyimilinganiselo yesibini ephambili yokukhetha i-pipette efanelekileyo kwibhubhoratri yakho. Iingcebiso zokucoca zixabisa kakhulu xa zithelekiswa neengcebiso zenjongo jikelele. Ke, ukuba uhlahlo lwabiwo-mali luqinile kwaye awuzimisele ukwenza naluphi na uvavanyo lwemolekyuli, ke ukuthenga iingcebiso zenjongo ngokubanzi lolona khetho lulungileyo.

I-Cotaus yincutshe yomenzi wamacebiso epipette kunye nomthengisi, ebonelela abathengi ngeenkcukacha ezahlukeneyo zeengcebiso zepipette. Imveliso nganye ihlangabezana neemfuno zomgangatho womthengi. Khetha iCotaus kukukhetha ukuchaneka kunye nokusebenza kakuhle.