Ngaba abahlobo abaselabhoratri bahlala bebhideka ngumahluko phakathi kweetyhubhu zePCR, iityhubhu zeEP, kunye netyhubhu ezisibhozo? Namhlanje ndiza kwazisa umahluko kunye neempawu zezi zintathu

Ngaba abahlobo kwilabhoratri bahlala bebhidekile ngumahluko phakathiityhubhu yePCRs, EP iityhubhu, kunye netyhubhu ezisibhozo? Namhlanje ndiza kwazisa umahluko kunye neempawu zezi zintathu

1. ityhubhu yePCR

ityhubhu yePCRs zidla ngokusetyenziswa ezisetyenziswayo kwimifuniselo yebhayoloji. Ngokomzekelo, iityhubhu zeCotaus®PCR zisetyenziselwa ukubonelela ngee-container ze-PCR (i-polymerase chain reaction) yovavanyo, enokuthi isetyenziswe kwi-mutation, i-sequencing, i-methylation, i-molecular cloning, i-gene expression, i-Genotyping, iyeza, i-forensic science kunye nezinye iinkalo. Umbhobho we-PCR oqhelekileyo uqulunqwe ngumzimba wetyhubhu kunye nekhava, kunye nomzimba wetyhubhu kunye nekhava zidibene kunye.

Isixhobo sokuqala sePCR sasingenaso isiciko esishushu. Ngexesha lenkqubo ye-PCR, ulwelo olusemazantsi ityhubhu luya kunyuka lube ngumphunga ukuya phezulu. Isigqubuthelo seconvex (oko kukuthi, umphezulu ongqukuva) sasiyilelwe ukuququzelela ukuphuphuma kolwelo ukujiya nokuqukuqela ukuhla. Nangona kunjalo, isixhobo sangoku sePCR ngokusisiseko luhlobo lwekhava eshushu. Ubushushu obuphezulu kwikhava ye-PCR buphezulu kwaye ubushushu ezantsi buphantsi. Ulwelo olusezantsi alululanga ukuba lube ngumphunga ukuya phezulu, ngoko ke uninzi lwazo lusebenzisa izigqubuthelo ezisicaba.

2. EP ityhubhu

Ngenxa yokuba ityhubhu ye-centrifuge yaqalwa kwaye yaveliswa ngu-Eppendorf, ikwabizwa ngokuba yi-EP tube.

Umahluko omkhulu phakathiityhubhu yePCRI-s kunye ne-microcentrifuge tubes kukuba iityhubhu ze-microcentrifuge ngokubanzi zineendonga zetyhubhu ezityebileyo zokuqinisekisa iimfuno ze-centrifugation, ngelixa.ityhubhu yePCRs babe iindonga ityhubhu obhityileyo ukuqinisekisa isantya transfer ubushushu kunye nokufana. Ngoko ke, ezi zimbini azikwazi ukuxutywa kwizicelo eziphathekayo, kuba iibhubhu ze-PCR ezincinci zinokugqabhuka ngenxa yokungakwazi ukumelana nemikhosi emikhulu ye-centrifugal; ngokufanayo, iityhubhu ze-microcentrifuge ezityebileyo ziya kuchaphazela isiphumo se-PCR ngenxa yokuhanjiswa kobushushu obucothayo kunye nokudluliselwa kobushushu obungalinganiyo.

3.iityhubhu ezisibhozo

Ngenxa yomthwalo onzima wokuvavanya i-batch kunye nokusebenza okungahambi kakuhle kombhobho enye, iibhubhu ezisibhozo kwimiqolo zenziwa.eeCotaus®I-PCR 8-strip tube yenziwe ngepolypropylene ephuma kumazwe angaphandle, kwaye isigqubuthelo setyhubhu sihambelana nomzimba wetyhubhu, onomsebenzi obalaseleyo wokutywinwa. Kwangaxeshanye, inokuguquguquka okuqinileyo kwaye inokuhlangabezana neenjongo ezahlukeneyo zokulinga.