Intambo yangaphakathi okanye intambo yangaphandle, indlela yokukhetha i-vials cryogenic?

Kwiimvavanyo zophando lwezenzululwazi, i-cryovials sisixhobo esibalulekileyo sokugcinwa kwexesha elide leeseli, i-microorganisms, iisampulu zebhayoloji, njl., Ukubonelela ngokuzinzileyo, ubushushu obuphantsi kwendawo yokugcina iisampulu zebhayoloji ukuqinisekisa umsebenzi kunye nokuthembeka kweesampulu.

Nangona kunjalo, xa sithatha iisampulu ezigcinwe ixesha elide kwifriji ye-ultra-low okanye itanki ye-nitrogen engamanzi, sihlala sothuswa ngesiquphe sisandi esiqhekezayo setyhubhu ye-cryogenic kwaye sibanjwe yintliziyo. Ukugqabhuka kweetyhubhu ze-cryovials akuyi kubangela kuphela ilahleko yeesampulu zovavanyo, kodwa kunokubangela ukwenzakala kubasebenzi bovavanyo.

Yintoni ebangela ukugqabhuka kweVial yokuGcina? Sikuthintela njani oku ukuba kungenzeki?

Oyena nobangela wokugqabhuka kwetyhubhu yesikhenkcezisi yintsalela yenitrogen yolwelo ngenxa yokuxinana komoya okulambathayo.Xa ityhubhu yesampulu ye-cryopreservation ikhutshwa kwitanki yenitrogen engamanzi, ubushushu ngaphakathi kumbhobho buyanda, kwaye initrogen elulwelo ekwityhubhu imka ngokukhawuleza kwaye iyatshintsha. ukusuka kulwelo ukuya kwirhasi. Ngeli xesha, ityhubhu ye-cryovials ayikwazi ukususa i-nitrogen engaphezulu kwexesha, kwaye iqokelela kwi-tube. Uxinzelelo lwenitrogen lukhula ngokukhawuleza. Xa umzimba wombhobho awukwazi ukujamelana noxinzelelo oluphezulu olwenziwe ngaphakathi, luya kugqabhuka, lubangele ukuqhuma kombhobho.

Ngaphakathi okanye ngaphandle?

Ngokuqhelekileyo sinokukhetha ityhubhu ye-cryovial ejikelezayo yangaphakathi enomoya olungileyo. Ngokumalunga nolwakhiwo lwesigqubuthelo setyhubhu kunye nomzimba wetyhubhu, xa i-nitrogen engamanzi kwityhubhu ye-cryovial ejikeleza ngaphakathi iphuphuma, kulula ukuyikhupha kunetyhubhu ye-cryovial ejikelezayo ngaphandle. Ngaphezu koko, umahluko woyilo womgangatho ofanayo weetyhubhu ze-cryogenic uya kubangela ukuba ityhubhu ye-cryopreservation ejikelezayo yangaphakathi ibe ngumphunga. Ukusebenza kokutywinwa kombhobho ogciniweyo kungcono kunombhobho odibeneyo wangaphandle, ngoko ke akunakwenzeka ukuba kubangele ukuqhuma kombhobho.

Ikepusi yangaphandle eneneni yenzelwe ukukhenkceza koomatshini, okwenza ukuba kufikeleleke kancinci kwisampulu ngaphakathi kwityhubhu kwaye ngaloo ndlela kuncitshiswe ukungcoliseka kwesampulu. Inokubekwa ngokuthe ngqo kwifriji yokukhenkceza, kwaye ayifanelekanga ukugcinwa kwenitrogen engamanzi.

Ityhubhu yeCotaus cryovials enekhowudi ezintathu:

I-1.I-tube cap kunye nomzimba wombhobho ziveliswa kwi-batch efanayo kunye nemodeli ye-PP yezinto ezibonakalayo, ngoko ke i-coefficient yokwandisa okufanayo iqinisekisa ukutywinwa nakweyiphi na iqondo lokushisa. Iyakwazi ukumelana nobushushu obuphezulu obuyi-121℃ kunye noxinzelelo oluphezulu lokuvala inzala kwaye inokugcinwa kwi- -196℃ kubume benitrogen engamanzi.

2. Ityhubhu ye-cryo ejikelezayo yangaphandle yenzelwe iisampulu zokukhenkceza. I-screw cap ejikelezayo yangaphandle inokunciphisa ithuba lokungcoliseka xa uphatha iisampuli.

3. Ii-cryovials ezijikelezayo zangaphakathi ziyilelwe iisampulu zokukhenkceza kwisigaba segesi yenitrogen engamanzi. I-silicone gasket emlonyeni wombhobho iphakamisa ukutywinwa kwe-cryovial.

4. Umzimba wetyhubhu unokukhanya okuphezulu kwaye udonga lwangaphakathi lulungiselelwe ukugalelwa lula kolwelo kwaye akukho ntsalela kwisampulu.

5. I-2ml ityhubhu ye-Cryovial ilungelelaniswe kumgangatho oqhelekileyo we-SBS plate rack, kwaye i-tube cap ngokuzenzekelayo ingalungiswa kwi-channel enye kunye ne-multi-channel automatic cap openers.

6. Indawo yokumakisha emhlophe kunye nesikali esicacileyo senza kube lula kubasebenzisi ukuphawula kunye nokulinganisa umthamo. Ukudityaniswa kwekhowudi ye-QR esezantsi, ikhowudi yebhakhowudi, kunye nekhowudi yedijithali yenza ulwazi lwesampulu lucace ngokukrwaqula, lunciphisa kakhulu umngcipheko wokubhideka kwesampulu okanye ilahleko.

I-Cotaus i-cryogenic vials ezintathu-in-one ziveliswa ekuqaleni kwi-polypropylene yonyango. Amandla akhoyo ngoku yi-1.0ml kunye ne-2.0ml, kunye nezinye iinkcukacha zingenziwa ngokwezifiso ngokweemfuno zabathengi. Ngokusebenza kwayo okugqwesileyo kunye noyilo olufanelekileyo, ibonelela ngokhetho olungcono kubaphandi besayensi. Nokuba ingaphakathi okanye yangaphandle, inokuhlangabezana neemfuno zakho zovavanyo ezohlukeneyo kwaye yenze indlela yakho yophando lwezenzululwazi ibe lula. Khetha iCotaus, yenza iziphumo zakho zovavanyo zibalasele ngakumbi!