Intshayelelo ye Elisa Plate

ELISA PLATE: Kwi-Enzyme edibeneyo ye-Immunosorbent Assay (ELISA), ukucoceka, ukugxininiswa kunye nomlinganiselo we-antigens, i-antibodies, i-antibodies ebhaliweyo okanye i-antigens echaphazelekayo kwi-immunological reaction; uhlobo lwe-buffer, ugxininiso kunye neeMeko ezifana namandla e-ionic, ixabiso le-pH, ubushushu bokuphendula kunye nexesha lidlala indima ebalulekileyo. Ukongezelela, umphezulu we-polystyrene yesigaba esiqinileyo (i-Polystyrene) njengomthwali nayo idlala indima ebalulekileyo kwi-adsorption ye-antigens, i-antibodies okanye i-antigen-antibody complexes.

I-Antigens, amajoni omzimba kunye nezinye ii-biomolecules zibhengezwa kumphezulu ophethe ngeendlela ezahlukeneyo, kubandakanywa i-adsorption passive ngokusebenzisa iibhondi ze-hydrophobic, iibhondi ze-hydrophobic / ionic, i-covalent bonding ngokungeniswa kwamanye amaqela asebenzayo afana namaqela e-amino kunye nekhabhoni, kunye nokuguqulwa komhlaba. . I-Hydrophilic bonding emva kwesondo.

IElisa Plateinokwahlulwa ibe ngama-48-kahle kunye nama-96-kakuhle ngokwenani lemingxuma. Eyona isetyenziswa ngokuqhelekileyo yi-96-well, ekufuneka ikhethwe ngokomfundi wakho we-microplate.

Ukongeza, kukho ezinokuthi zisuswe kwaye zingenakucinywa. Kwizinto ezingabonakaliyo, ii-slats kwibhodi yonke zidibene kunye. Emva koko, kwii-detachable, ii-slats ebhodini ziyahlukana, kwaye iibhodi ezihlukeneyo Kukho i-12-hole kunye ne-8-hole strips. Ngokuqhelekileyo, iipleyiti ezinophawu lwe-enzyme ezinokuthi zifumaneke zisetyenziswa ngokuqhelekileyo namhlanje. Ukuba uthenge iipleyiti ezinjalo ngaphambili, ungathenga nje imicu ebhalwe nge-enzyme ngoku.

Nangona ii-microplates ezenziwe ngabavelisi abahlukeneyo zibukeka zifana ngokubanzi, ezinye iinkcukacha ezincinci ziya kuba zihluke, ezifana nesakhiwo, njl. Oku ikakhulu kuba kufuneka zisetyenziswe ngabafundi abahlukeneyo be-microplate. Ngoko ke, xa usebenzisa Xa ukhetha ukuthenga i-microplate reader, kufuneka uphinde ucinge ukuba umfundi wakho we-microplate ukhangeleka njani. Kodwa ngokubanzi zihlengahlengiswa, kuphela ezinye ziya kwahluka. Ngenxa yokuba izinto zepleyiti ye-enzyme ngokuqhelekileyo ziyi-polystyrene (PS), kwaye i-polystyrene inozinzo olubi lwekhemikhali kwaye inokunyibilika ngeentlobo ezahlukeneyo ze-solvents eziphilayo (ezifana ne-hydrocarbons enevumba elimnandi, i-halogenated hydrocarbons, njl. njl.), kwaye iya kugqweswa yi-acids eqinile. kunye neealkali. , ayixhathisi igrisi, kwaye kulula ukuyitshintsha umbala emva kokuvezwa kukukhanya kwe-ultraviolet, ke qiniseka ukuba uhoye oku xa usebenzisaElisa Plate.