Kutheni le nto izinto ezityiwayo zePCR zisenziwa ngokubanzi ngePP?

"Njengoko sonke siyazi, i-PCR yindlela esisiseko yovavanyo kwiilabhoratri ze-biochemical." Iziphumo zovavanyo zihlala zinganelisekanga, ezinokuthi zibe ngenxa yokungcoliseka okuncinci kweplastiki ye-PCR, okanye ukuphazamiseka kovavanyo okubangelwa ukuqaliswa kwee-inhibitors. Kukho esinye isizathu esibaluleke kakhulu: Ukukhetha okungafanelekanga kwezinto ezisetyenziswayo kuya kuba nefuthe elikhulu kwiziphumo zovavanyo.

Zininzi izizathu ezichaphazela iziphumo zovavanyo lwe-PCR: ngokuqhelekileyo kukho ezi ntlobo zi-7 zilandelayo.

1. I-Primers: I-Primers iyisitshixo kwi-reaction ethile ye-PCR, kwaye ukuchaneka kweemveliso ze-PCR kuxhomekeke kwinqanaba lokuhambelana phakathi kwee-primers kunye ne-template DNA;

2. I-Enzyme kunye nokugxila kwayo;

3. Umgangatho kunye nogxininiso lwe-dNTP;

4. I-template (i-target gene) i-nucleic acid;

5. Ugxininiso lweMg2+;

6. Ukumiselwa kobushushu kunye nexesha;

7. Inani lemijikelo;

8. Izixhobo, izinto ezisetyenziswayo, njl.

Phakathi kwezinto ezininzi ezinempembelelo, izinto ezisetyenziswayo yenye yezinto ezibaluleke kakhulu nezingahoywa ngokulula.

Kukho iintlobo ezininziPCR izinto ezisetyenziswayo: Iityhubhu ezi-8, iibhubhu ezisezantsi, iibhubhu eziqhelekileyo, ezinqabileyo, ezinqamlekileyo, ezinqamlekileyo, ezigcweleyo, kunye neengcingo ze-PCR kunye ne-qPCR. Kunzima kakhulu ukukhetha, kwaye zininzi iingxaki eziqhelekileyo, makhe sijonge iingxaki ezikhethwa ngumntu wonkePCR izinto ezisetyenziswayo, nendlela yokuyicombulula?

KutheniPCR izinto ezisetyenziswayoyenziwe ngokubanzi ngePP?

Impendulo: Izinto ezisetyenziswayo ze-PCR / qPCR ngokuqhelekileyo zenziwe nge-polypropylene (PP), ngenxa yokuba i-biologically inert material, i-surface akulula ukunamathela kwi-biomolecules, kwaye inokumelana neekhemikhali ezilungileyo kunye nokunyamezela ukushisa (inokuthi i-autoclaved kwi-121 degrees) ibhaktheriya. kwaye inokumelana nokutshintsha kobushushu ngexesha lokuhamba ngebhayisekile eshushu). Ezi zixhobo zihlala zidibanisa ngokuthe ngqo kunye nee-reagents okanye iisampuli, ngoko ke izinto ezisemgangathweni ophezulu kunye neendlela ezifanelekileyo zokucwangcisa kufuneka zikhethwe ngexesha lokuvelisa kunye nenkqubo yokulungiselela.