Ukusetyenziswa okuqhelekileyo kweePlati ze-ELISA

Njengesixhobo sokulinga, isakhiwo esingundoqo seELISA ipleyitiluluhlu lwee-microplates eziqulethe izinto eziqinileyo zesigaba (ezifana neeprotheni kunye nezilwa-buhlungu). Ekusetyenzisweni kwepleyiti ye-ELISA, isampuli eya kuvavanywa iya kusabela nge-molecule ethile ebhalwe nge-enzyme, kwaye emva koko utshintsho olubonakalayo lombala luya kuveliswa ngokudibanisa i-matrix substrate, kwaye umxholo okanye umsebenzi we-molekyuli ekujoliswe kuyo iya kubalwa. okanye ivandlakanywe ngokubona i-absorbection okanye isignali ye-fluorescence. Oku kulandelayo kukusetyenziswa okuqhelekileyo kweepleyiti ze-ELISA kwiinkalo ezahlukeneyo:

1. Uhlalutyo lwamanani eprotheyini: Iipleyiti ze-ELISA zingasetyenziselwa ukulinganisa ukugxininiswa kunye nomsebenzi weeprotheni kwiisampuli zebhayoloji ezifana ne-serum kunye ne-cell supernatants, ukubonelela ngezixhobo ezinamandla zokubona iimpawu ze-tumor, i-hepatitis virus antibodies, iimpawu zokulimala kwe-myocardial, njl. kunye nokuncedisa oogqirha ekuxilongeni kwangethuba nasekuvavanyeni izifo.

2. Ukujongwa kwe-cytokine: Kuphando lwe-immunology,Iipleyiti ze-ELISAinokulinganisa amanqanaba e-cytokine kwi-cell culture supernatants okanye i-tissue fluids, enceda ukuqonda iinkqubo ze-biological ezifana neempendulo ze-immune kunye neempendulo ezivuthayo, kwaye kubaluleke kakhulu ekuphuhliseni unyango olutsha kunye neziyobisi.

3. Uphando lwe-Nucleic acid: Ngokusebenzisa iipleyiti ze-ELISA, izazinzulu zinokufumanisa kwaye zihlalutye umxholo kunye nomsebenzi we-DNA okanye i-RNA, ukubonelela ngenkxaso yedatha yophando lwebhayoloji ye-molekyuli efana ne-gene expression kunye nokulawulwa kofuzo, kwaye ikhuthaze ngakumbi ukuphuhliswa kwemimandla efana nonyango lwemfuza. kunye nokuhlelwa kofuzo.

4. Uphando lomsebenzi we-Enzyme: Iipleyiti ze-ELISA zinokulinganisa ngokuchanekileyo umsebenzi we-enzyme, banceda abaphandi baqonde umsebenzi kunye nokulawula indlela yokulawula i-enzymes kwizinto eziphilayo, kunye nokubonelela ngeengxelo ezibalulekileyo zophando kubunjineli be-enzyme, ubunjineli be-metabolic kunye nezinye iinkalo.

5. Uphando lwentsebenziswano phakathi kweemolekyuli:Iipleyiti ze-ELISAingasetyenziselwa kuphela ukulinganisa umxholo weemolekyuli, kodwa nokufunda intsebenziswano phakathi kweeathom. Ngokudibanisa iitekhnoloji ezifana ne-surface plasmon resonance kunye ne-fluorescence resonance energy transfer, inkqubo yokubophelela kunye nokuhlukana phakathi kweeamolekyu inokujongwa ngexesha langempela, ukubonelela ngemibono emitsha kunye neendlela zokwenza iziyobisi, ukusebenzisana kweprotheni kunye nolunye uphando.