Indima yeMibhobho yeChemiluminescent

Indima yeiityhubhu ze-chemiluminescentubukhulu becala bubonakaliswa kubuchule babo bokuguqula amandla akhutshiweyo kwiikhemikhali abe ngamandla okukhanya, ngaloo ndlela ekhupha ukukhanya okubonakalayo okanye ukukhanya kobude obuthile. Le nkqubo yokuguqula yenza iityhubhu ze-chemiluminescent zidlale indima ebalulekileyo kwiinkalo ezininzi, kuquka le miba ilandelayo:

1. Ukufunyanwa kunye nohlalutyo:

Ukusetyenziswa ngokuthe ngqo kweetyhubhu ze-chemiluminescent njengesixhobo sokufumanisa. Ngokuyila i-reaction yekhemikhali ethile, isabela kunye nento eza kuvavanywa kwaye ivelise isibonakaliso se-luminescent, ngaloo ndlela iqonda ukufunyanwa kwento ekujoliswe kuyo. Le ndlela yokufumanisa inovakalelo oluphezulu kunye neenkcukacha eziphezulu, kwaye iyakwazi ukubona izinto eziphantsi kakhulu, nakwinqanaba le-molecule enye.

Kwintsimi ye-biomedicine, iityhubhu ze-chemiluminescent zingasetyenziselwa ukufumanisa iimolekyuli ze-biological ezifana neeprotheni, i-nucleic acids, i-hormone, kunye neziyobisi kwiisampuli ze-biological, ukubonelela ngesiseko esibalulekileyo sokuxilongwa kwangaphambili kwezifo, ukuhlolwa kweziyobisi kunye novavanyo lwemiphumo yonyango.

Kwinkalo yenzululwazi yendalo esingqongileyo, iibhubhu ze-chemiluminescent zingasetyenziselwa ukufumanisa ukungcola emanzini kunye ne-atmosphere, njenge-ion yensimbi enzima, ukungcola kwezinto eziphilayo, njl., eziluncedo ekukhuselweni kwendalo kunye nokulawula ukungcola.

2. Uhlalutyo lomgangatho kunye nobungakanani:

Iityhubhu zeChemiluminescentayikwazi ukubona kuphela ubukho okanye ukungabikho kwezinto ekujoliswe kuzo, kodwa kwakhona ukwenza uhlalutyo lobungakanani ngokulinganisa ubunzulu beempawu zokukhanya. Ubukhulu besignali ye-luminescent idla ngokunxulumana ngokuhambelana noxinaniso lwento ekujoliswe kuyo, ngoko ke uxinaniso lwento ekujoliswe kuyo lunokuchazwa ngokulinganisa ubunzulu bokukhanya.

3. Ukujongwa kwexesha lokwenyani kunye nokubonwa ngokukhawuleza:

Iityhubhu ze-Chemililuminescent zineempawu zesantya sokuphendula ngokukhawuleza kunye nokusebenza okulula, kwaye zifanelekile ukujonga ixesha langempela kunye nokufumanisa ngokukhawuleza. Kwiimeko apho iziphumo zovavanyo kufuneka zifumaneke ngokukhawuleza, njengokuhlangula ngokukhawuleza, ukuvavanywa kokhuseleko lokutya, njl., iibhubhu ze-chemiluminescent zinokubonelela ngokukhawuleza iziphumo zovavanyo oluthembekileyo.

4. Ukwandiswa komqondiso kunye nokwandiswa:

Kwezinye iimeko, ukwenzela ukuba kuphuculwe ngakumbi uvakalelo lokubhaqa, imiqondiso ye-chemiluminescent inokwandiswa kwaye iphuculwe ngokusebenzisa iikhemikhali ezithile okanye iindlela zobugcisa. Oku kunceda ukufumanisa izinto ezijoliswe kuzo kwiindawo ezisezantsi kunye nokwandisa uluhlu lwezicelo zeetyhubhu ze-chemiluminescent.

5. Ubhaqo lwamacandelo amaninzi:

Ngokuyila iinkqubo ezahlukeneyo zokusabela kweekhemikhali okanye ukusebenzisa ii-reagents zokukhanya ezahlukeneyo,iityhubhu ze-chemiluminescentinokufikelela ekubhaqweni ngaxeshanye kwamacandelo amaninzi. Oku kubaluleke kakhulu kuhlalutyo lweesampulu ezinzima kwaye kunokuphucula kakhulu ukufumanisa ukusebenza kakuhle kunye nokuchaneka.