Isikhokelo kwiiNgcebiso zePipette zeLebhu ezahlukeneyo

Ziziphi iingcebiso zepipette?

Iingcebiso zePipette zizixhobo ezilahlayo kwiipayipi ezisetyenziselwa ukuhambisa ulwelo ngokuchanekileyo. Ziza ngokobukhulu obahlukeneyo, imathiriyeli, kunye neentlobo, ezinje ngomgangatho, ukuncamathela okuphantsi, ukuhluzwa, kunye neengcebiso zobude obude.

Iingcebiso zikaPipette zibalulekile kwiilebhu zesayensi kwaye zisetyenziswa ngokubanzi kwisayensi yobomi, ikhemistri, amayeza, i-biotechnology, kunye nebhayoloji yemolekyuli. Ngenxa yezicelo zabo ezahlukeneyo, amaqumrhu alawulayo kwihlabathi jikelele abeka imigangatho yomgangatho ukuqinisekisa ukuchaneka nokuthembeka kuphando. UCotaus, umenzi owaziwayo wezinto ezityiwayo zebhayoloji eTshayina, uvelisa iingcebiso ezikumgangatho ophezulu wepipette eziqinisekisiweyo yi-ISO, CE, kunye ne-FDA eqinisekisiweyo, eqinisekisa ukuthembeka kunye nokuthotyelwa kophando lwezenzululwazi.

Namhlanje, makhe sihlolisise iintlobo ezahlukeneyo zeengcebiso ze-pipette, ukuqonda iimpawu zabo ezikhethekileyo kunye nokusetyenziswa kwindlela yokuphatha ulwelo oluchanekileyo.

Iindidi ezahlukeneyo zeengcebiso zepipette

1. Iingcebiso zePipette ezisemgangathweni (kwi-Universal).

Iingcebiso ze-pipette eziqhelekileyo, ezikwabizwa ngokuba ziingcebiso zendalo yonke, zihlala zenziwe ngekhwalithi ephezulu, i-autoclavable polypropylene. Ziyezona ntlobo zixhaphakileyo ze-pipette accessory ezisetyenziswa kwiilabhoratri ezineemfuno ezahlukeneyo zokusebenza ezisuka ekuchanekeni okuphezulu ukuya kwi-reagent dispensing ngonyamezelo olukhulu, eyenzelwe ukulungelelanisa uluhlu olubanzi lweempawu ze-pipette kunye neemodeli, ezizenza zibe ziguquguqukayo kwaye zilungele ulwelo jikelele. ukuphatha imisebenzi. Ifumaneka kuzo zombini iinguqulelo ezinyumba kunye nezingazaliyo ngokuxhomekeke kwiimfuno ezithile zovavanyo.

IiNgcebiso eziNgaNzalanga vs

Iingcebiso ezingaNzala:Ezi zingasetyenziselwa iinkqubo zelebhu jikelele apho ubunyumba bungabalulekanga. Ziyindleko-esebenzayo kwimisebenzi yesiqhelo okanye iisampulu ezingavakaliyo.

Iingcebiso eziNdlolo: Zibalulekile kwizicelo ezibuthathaka ezifana ne-microbiology, i-molecular biology, kunye novavanyo lweklinikhi, njengoko zifakwe inzala kwangaphambili kwaye ziqinisekiswa ukuba azinangcoliseko ezifana ne-RNase, DNase, kunye ne-endotoxins njl. njl. kodwa i-autoclaving inokuphelisa umngcipheko wongcoliseko olubangelwa zizidalwa eziphilayo, oku akuthethi ukuba iingcebiso ziya kukhululeka. I-RNase kunye ne-DNase.

Ukuba ufuna ukwenza iimvavanyo ezinovakalelo apho oku kufuneka khona, kufuneka ukhethe iingcebiso ze-pipette eziyinyumba ukusuka kumenzi onokuqinisekisa ukuba iingcebiso zabo azikho kwi-RNase kunye ne-DNase.

eCotausiingcebiso eziqhelekileyoziza ngokwesayizi ezahlukeneyo zevolumu (umzekelo, 10 µL, 20 µL, 50 µL, 100 µL, 200 µL, 300 µL, 1000 µL).

2. Isihluzo vs Iingcebiso ezingezizo Isihluzi

Iingcebiso zesihluzi:Iingcebiso ezihluziweyo zibonisa umqobo omncinci, owenziwe ngokuqhelekileyo kwizinto eziphathekayo ze-hydrophobic, ezingaphakathi kwincam. Esi sihluzo sithintela ukungcoliseka phakathi kweesampuli kunye ne-pipette. Iingcebiso zesihluzi zenzelwe ukusetyenziswa kwiintlobo ezithile zovavanyo. Ngokomzekelo, ukuba isampuli i-corrosion, i-volatile, okanye i-viscous kakhulu kwindalo, inokubangela ukonakalisa i-pipette. Kwiimeko ezinjalo, iingcebiso zokucoca ziyacetyiswa.

Ngalo lonke ixesha unqwenela ulwelo, ii-aerosols ziveliswa ngaphakathi kwincam yepipette. Ukuba awusebenzisi iingcebiso zokucoca, ezi aerosols zinokuthi zingcolise ipipette yakho kunye neesampuli ezilandelayo, ezichaphazela iziphumo zakho zovavanyo. Ke ngoko, iingcebiso zokucoca zineendleko eziphezulu kwimifuniselo echanekileyo.

Iingcebiso ezingahluziyo:Iingcebiso ezingahluziyo zizona ngcebiso ze-pipette ezisetyenziswa ngokuqhelekileyo kwiilabhoratri njengoko zibiza kakhulu kuneengcebiso zokucoca. Zifaneleke kakhulu kwiisampuli ezingenakulungele ukungcoliseka kwaye akunakwenzeka ukulimaza i-pipette. njengokwahlula i-plasmid DNA, kunye nokulayisha iijeli ze-agarose, phakathi kwezinye. Nangona kunjalo, abanalo izibonelelo zokuthintela ukungcoliseka kweengcebiso zokucoca, zibenza bangalungeli kangako imifuniselo ebalulekileyo okanye enovakalelo.

3. Ugcino oluPhantsi vs Iingcebiso zokuGcina okungaPhantsi (Umgangatho)

Iingcebiso zepipette zokugcina eziphantsiziyilelwe ngokukodwa ukunciphisa ukugcinwa kolwelo ngaphakathi kwincam, iqinisekisa ukudluliselwa kwesampulu echanekileyo kunye nesebenzayo. Ezi ngcebiso zilungele ukusebenza nge-viscous, incangathi, okanye ulwelo oluxabisekileyo apho ukunciphisa ilahleko yesampulu kubalulekile. Nangona kunjalo, zibiza kakhulu kuneengcebiso eziqhelekileyo, ezi ngcebiso zilungele ukuqokelela iisampuli ngexesha le-PCR, ukuhlanjululwa kweprotheyini, i-SDS-PAGE, i-cloning, i-DNA kunye ne-RNA izicelo kunye nezicelo ezahlukeneyo zokuhlalutya iiprotheyini.

4. Iingcebiso ezimfutshane vs. Ubude obandisiweyo

Iingcebiso ezimfutshane zepipetteziyilelwe ukusetyenziswa kwiipleyiti ezininzi kakuhle, ezifana 1536 okanye 384-ifomathi kakuhle, apho ubungakanani babo encinane inceda ekujoliswe emxinwa amaqula ngokuchanekileyo. Ezi ngcebiso ziphinda ziphucule i-ergonomics ngokuvumela i-pipetting kufuphi nebhentshi, ukunciphisa ukuxhatshazwa kwengalo ngexesha lemisebenzi ephindaphindiweyo. Ilungele ukuhlolwa okuphezulu kunye nokuphucula induduzo yebhubhoratri.

Iingcebiso zepipette zobude obudezinde kuneengcebiso eziqhelekileyo, zibonelela ngolawulo olungcono longcoliseko ngokuvumela ukufikelela ngaphantsi kweenqanawa ngelixa unciphisa ukudibana nesikhongozeli. Ezi ngcebiso zilungele ukusetyenziswa kunye nezixhobo zaselebhu ezifana neebhloko zequla elinzulu kunye neityhubhu ze-microcentrifuge, eziqinisekisa ukuphathwa kolwelo oluchanekileyo kwiindawo ekunzima ukufikelela kuzo.

5. Iingcebiso zePipette ezibanzi

Iingcebiso zepipette ezibanzibonisa isiphelo esikude kunye ne-orifice ukuya kuthi ga kwi-70% enkulu kuneengcebiso eziqhelekileyo, uphawu luluncedo ngokukodwa ekupheliseni ukucheba kweeseli kunye nokumelana nokuhamba. Ukuzenza zilungele ukuphatha iisampulu ezinzima ukuya kwipipette njengemigca yeeseli ezibuthathaka, i-genomic DNA, i-hepatocytes, i-hybridomas, kunye nolunye ulwelo olubonakalayo. Ezi ngcebiso zinciphisa amandla okucheba ngoomatshini, ukuthintela ukuqhekeka kweeseli kunye nokuqinisekisa ukusebenza kakuhle kweeseli kunye nokusebenza kakuhle kweplating.

6. Iingcebiso ze-Robotic Pipette

Iingcebiso ze-robotic pipetteziyilelwe ukusetyenziswa ngeentlobo ngeentlobo zeenkqubo zokuphatha ulwelo oluzenzekelayo kunye neerobhothi zombhobho. Ezi ngcebiso ziqinisekisa ukuhambelana neempawu (Hamilton, Beckman, Agilent, eeTecan, njl.) kwi-automation yebhubhoratri, ukuphucula ukuchaneka kunye nokusebenza kakuhle kwizicelo eziphezulu. Iingcebiso zeRobhothi zilawulwa phantsi kokunyamezela okunamandla xa kuthelekiswa neengcebiso ze-pipette manual. Ezi ngcebiso ze-auto-robotic ziqinisekisa ukuchaneka okuphezulu, ukuchaneka, kunye nokusebenza kakuhle kwizicelo eziphezulu zokusebenzisa kwiinkalo ezahlukeneyo, kubandakanywa i-genomics, i-proteomics, kunye nophando lwamayeza.

Umzekelo:

Iingcebiso zepipette eziqhubayoziingcebiso ezikhethekileyo ezisetyenziswa kwiinkqubo zombhobho ezizenzekelayo eziyilelwe ukunciphisa i-electrostatic charge buildup ngexesha lokuphatha ulwelo. Ezi ngcebiso ziyimfuneko kwizicelo apho uphazamiseko lwe-electrostatic lunokuchaphazela imfezeko yesampulu okanye ukuchaneka kweenkqubo zokuphatha ulwelo oluzenzekelayo.

7. Iingcebiso ezikhethekileyo zePipette

Izicelo ezithile zifuna uyilo lweengcebiso ezizodwa zepipette kwimisebenzi ethile.

Imizekelo:

PCR iingcebiso:Iingcebiso ezilungiselelwe ukusetyenziswa kwiinkqubo ze-polymerase chain reaction (PCR) ukukhusela ungcoliseko olusuka kwi-DNA eyandisiweyo.
Iingcebiso zeCryogenic:Yenzelwe ngokukodwa ukusetyenziswa namaqondo obushushu asezantsi kakhulu kwaye ihlala ingena kulwakhiwo olomeleleyo, oluluqilima lokuphatha iisampulu ezinomkhenkce.

Ukuqukumbela

Ukukhethwa kweengcebiso ze-pipette kuxhomekeke kubume bovavanyo kunye nohlobo lwe-pipette esetyenziswayo. Nokuba yeyokuphatha ulwelo ngokubanzi, ukuthintela ukungcoliseka, okanye ukusebenza ngeesampulu ezithambileyo okanye ezibizayo, ukuqonda iintlobo kunye neempawu zeengcebiso ze-pipette kuqinisekisa ukuhanjiswa kolwelo oluchanekileyo kunye olusebenzayo kwilabhoratri. Njalo khetha ingcebiso efanelekileyo ye-pipette kwiimfuno zakho zophando oluthile ukuze uqinisekise iziphumo ezilungileyo kunye nokuthembeka.