Kutheni izitya zenkcubeko yeseli zifuna unyango olungaphezulu?

Ukuze kuhlangatyezwane neemfuno zovavanyo lwenkcubeko yeseli kunye nokufezekisa iziphumo ezinqwenelekayo, siqhele ukusebenzisa unyango lwe-TC, unyango oluphuculweyo lwe-TC kunye nonyango lokuncamathela olusezantsi kakhulu kwiiseli ezinqunyanyisiweyo.

1. Unyango lwe-TC, olufanelekileyo kwinkcubeko yeeseli ezibambelelayo

Ngonyango olukhethekileyo lwe-plasma yegesi ye-vacuum, i-surface layer inokuthi ihlawulwe ngokuqhubekayo kwaye ihlawuliswe ngokulinganayo kunye namaqela amabini afanelekileyo kunye namabi ixesha elide, eliqinisekisa ukuxhamla kweeseli ezifanayo kunye nezinzile. Ukuqaliswa kwentlawulo ephindwe kabini kwenza i-TC surface ye-endothelial, i-hepatocyte kunye nenkcubeko yeseli ye-neuron ibe nokunamathela okungcono kunye nokusabalalisa kuneendawo ezifanayo ze-TC, kwaye inokufikelela ekusebenzeni ngokufanelekileyo kokubambelela kweeseli ukuhlangabezana nenkcubeko yomgangatho ophezulu weeseli ezihambelanayo. Umphezulu unokufezekisa ukusebenza kakuhle kokuncamathela kweeseli kunye nokuhlangabezana nenqanaba eliphezulu lenkcubeko yeseli enamathelayo.

2. Unyango olomeleziweyo lwe-TC, olulungele iiNkcubeko zeSeli ezineeMfuno eziPhezulu zokuBamba

Unyango oluphezulu lwenkcubeko ye-tissue, xa kuthelekiswa neemveliso eziqhelekileyo eziphathwe nge-TC, indawo ephuculweyo ye-TC inamandla okukhuthaza ukunamathela kweseli kunye nokwandiswa, ukwanda ngokukhawuleza kweeseli zeeseli, zingasetyenziselwa ukusasazwa kweeseli ezifunwayo, ezifana neeseli eziphambili okanye ezinobuthathaka, kunye neeseli ezikhuliswe phantsi kweemeko zokukhula ezithintelweyo (i-serum-free okanye i-serum encitshisiweyo), ukwanda ngokukhawuleza kwamanani eeseli, ukukhuthaza ukunamathela kweeseli kunye nokwandiswa, kunokugcinwa kwiqondo lokushisa kwegumbi ukwenzela uluhlu olubanzi lwezicelo.

3. Uluhlu lwe-adsorption oluphantsi kakhulu lwenkcubeko yokumiswa kweeseli

Ipolymer ekhethekileyo yeamphoteric yemolekyuli igqunywe kumphezulu wenqanawa yenkcubeko. Kuba le khompawundi ingakumbi i-hydrophilic, iimolekyuli ze-amphoteric zinokubhengeza iimolekyuli zamanzi ukuze zenze udonga lwamanzi, ukuze iiseli, iimolekyuli zeprotheyini, iibhaktheriya kunye nezinye izinto zingakwazi ukubambelela kwisitya senkcubeko, okukhokelela ekubambeleleni kweeseli ezisezantsi kakhulu ingakhuliswa ngokunqunyanyiswa ngaphezu kweentsuku ezili-15.

Ingasetyenziselwa inkcubeko yeeseli zomntwana, i-hemocyte kunye nezinye iiseli ezifuna ukukhula kwindawo yokunqunyanyiswa kwenkcubeko, kunye nokukhuthaza inkcubeko yeeseli ze-spheroid ze-3D kunye ne-organoids, kwaye ineempawu ezichasayo zokunamathela kwiiseli ezincamathelayo ezomeleleyo.

I-Cotaus inikezela ngoluhlu olupheleleyo lweemveliso zenkcubeko yeeseli, ngolwazi olungakumbi lwemveliso, nceda uqhagamshelane nathi.