Ukufika Okutsha | IYATHENGISWA | Black Elisa Plates

Kwiimeko ezahlukeneyo kwiinzululwazi zobomi, ukuzimisela kwexesha, okusebenzayo kunye noqoqosho kunye nobungakanani be-antigens okanye i-antibodies ekhoyo kwisampuli yinto ebalulekileyo.

I-Enzyme-linked immunosorbent assay (ELISA) ibonakalise ukuba luphando oluxabisekileyo kunye nesixhobo sokuxilonga sokulinganisa izilwa-buhlungu okanye ii-antigens kwiisampulu zebhayoloji nge-adsorption ye-antigens eyaziwayo okanye i-antibodies kumphezulu womphathi wesigaba esiqinileyo, esivumela i-enzyme ( ubukhulu becala HRP)-ebhalwe ii-antigen-antibody reactions kumphezulu wesigaba esiqinileyo. Obu buchule bunokusetyenziswa ukufumanisa iiantigens zemolekyuli enkulu kunye nezilwa-buhlungu ezithile, njl. njl. Ineenzuzo zokukhawuleza, uvakalelo, ulula, kwaye umthwali kulula ukulinganisa. Nangona kunjalo, ubuntununtunu kunye noluhlu oluguquguqukayo lokufunyanwa kwe-ELISA lukhawulelwe kakhulu kukungaphumeleli kwendlela yokufunxa ukukhanya ngenxa yempembelelo enkulu yeemeko zangaphandle kutshintsho lombala wesisombululo kunye noluhlu olusezantsi olusebenzayo lomgca wexabiso le-OD.

Itekhnoloji ye-DELFIA-- kukutshintsha ngokulula i-enzyme ye-HRP kunye ne-lanthanide chelate (Eu, Sm, Tb, Dy) ebhalwe kwi-antibody yokufumanisa kwiimvavanyo zemveli ze-ELISA. I-lanthanides esetyenziswa kwi-DELFIA ludidi olukhethekileyo lweziqalelo ze-fluorescent, ezibeka iimfuno kwizinto zovavanyo --- iipleyiti zikaElisa. I-Lanthanides inobomi be-fluorescence be-microseconds okanye i-milliseconds, ethi idityaniswe nokuchongwa kwexesha elixazululwe kunciphisa kakhulu ukuphazamiseka kwemvelaphi ye-autofluorescence, kunye nokutshintsha kwe-Strokes zabo ezibanzi kuphucula kakhulu uvakalelo lwe-assay.

Uninzi lwe-ELISA lukhetha ipleyiti yokulebula ye-enzyme ecacileyo njengomphathi kunye nesikhongozeli, kodwa ukukhanya okukhutshwe kwi-luminescence reaction yi-isotropic, ukukhanya akuyi kusasazwa ukusuka kwicala elithe nkqo, kodwa kuya kusasazwa ukusuka kwicala elithe tye, kwaye kuya kuthi. lula ukudlula umsantsa phakathi imingxunya ezahlukeneyo ipleyiti enzyme transparent kunye nodonga imingxuma Holes.Neighboring imingxuma basebenzisane omnye komnye kwaye kuchaphazela iziphumo zovavanyo.

Iipleyiti ze-Elisa eziMhlophe zingasetyenziselwa ukukhangela ukukhanya okubuthathaka kwaye ziqhele ukusetyenziselwa i-chemiluminescence jikelele kunye nophuhliso lombala we-substrate (umzekelo, uhlalutyo lwegene ye-luciferase ye-reporter).

Iipleyiti ze-Elisa eziMnyama eziMhlophe zinophawu olubuthathaka kuneepleyiti ezilebhile ze-enzyme emhlophe ngenxa yokufunxa ukukhanya kwazo, kwaye ziqhele ukusetyenziselwa ukubona ukukhanya okunamandla, okufana nokubhaqwa kwe-fluorescence.

Izinto ezilungileyo zeCotaus®Elisa Plates

● Ukubophelela okuphezulu

Iipleyiti zeCotaus®Elisa ezinetyhubhu emnyama zenziwe ngezinto ezingezizo ezizikhanyelayo, umphezulu uye wanyangwa ukuze uphucule kakhulu umthamo wawo wokubopha iprotheyini, enokufikelela kwi-500ng IgG/cm2, kwaye ubunzima bemolekyuli yeeprotheyini ezibotshelelweyo zi> 10kD. .

● I-fluorescence esezantsi isusa iingxaki ezibangwa ziimpendulo ezingangqalanga.

iityhubhu ezimnyama zinokususa uphazamiseko olungephi lwemvelaphi ye-fluorescence kuba iya kuba nokukhanya kwayo.

● Uyilo olunokususwa

Uyilo olunokudityaniswa lwesakhelo sepleyiti ye-enzyme emhlophe kunye ne-slats ye-enzyme emnyama ilungele ngakumbi ukusebenza. Nika ingqalelo kwisenzo sokuqhaqha, unganyanzeli ukuphula kwelinye icala, ngaphandle koko kuya kuba lula ukuphuka.

Ukuhlelwa kweMveliso

Ukufumana iinkcukacha ezingaphezulu zemveliso, nceda uzive ukhululekile ukuqhagamshelana nathi